Journal: iScience

Article Title: Chronic HDM exposure shows time-of-day and sex-based differences in inflammatory response associated with lung circadian clock disruption

doi: 10.1016/j.isci.2023.107580

Figure Lengend Snippet: Serum levels of total and HDM-specific immunoglobulins show a time-of-day response and sex-based differences to chronic HDM exposure Total IgE, Total IgG, HDM-specific IgE, HDM-specific IgG, HDM-specific IgG1, HDM-specific IgG2b, HDM-specific IgM, and HDM-specific IgA in the serum of chronic PBS- and HDM-exposed females and males at ZT0 and ZT12 were determined by ELISA. Data were expressed as ng/ml and mg/ml for total IgE and total IgG, respectively. HDM-specific immunoglobulins (IgE, IgG, IgG1, IgG2b, IgM, and IgA) in the serum were determined by commercially available ELISA kits (Chondrex, Inc.). Unable to detect HDM-specific IgG2a and IgG3 levels in PBS- and HDM-exposed mice. Data for the HDM-specific immunoglobulins were expressed as absorbance at 450 nm or μg/ml. Data are shown as mean ± SEM, Two-way ANOVA followed by Tukey’s multiple comparison test (n = 5/group). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, compared to respective control (PBS) at ZT0 or ZT12; # p < 0.05, compared to HDM at ZT0 vs. ZT12; # # p < 0.01, compared to HDM at ZT0 vs. ZT12. Summary statistics for interaction between Treatment x Sex x Time were analyzed using generalized linear modeling using R (see Table S2 ).

Article Snippet: Mouse Anti-House Dust Mite (HDM) IgG2b Antibody Assay Kit , Chondrex, Inc. , Cat# 3035.

Techniques: Enzyme-linked Immunosorbent Assay, Comparison, Control

Journal: iScience

Article Title: Chronic HDM exposure shows time-of-day and sex-based differences in inflammatory response associated with lung circadian clock disruption

doi: 10.1016/j.isci.2023.107580

Figure Lengend Snippet:

Article Snippet: Mouse Anti-House Dust Mite (HDM) IgG2b Antibody Assay Kit , Chondrex, Inc. , Cat# 3035.

Techniques: Purification, Recombinant, Staining, Enzyme-linked Immunosorbent Assay, Software

Journal: Scientific Reports

Article Title: Activation of GPR35 protects against cerebral ischemia by recruiting monocyte-derived macrophages

doi: 10.1038/s41598-020-66417-8

Figure Lengend Snippet: Pamoic acid (PA) activates Akt and p38 MAPK signaling in the ischemic brain. ( A ) PA treatment increased the concentration of pAkt at 24 hours after the MCAO, One-Way ANOVA, F (2/18) = 9.515, **P = 0.0039 (Bonferroni multiple comparison tests), values are min to max. n = 7. ( B ) PA treatment increases the pGSK-3β at 24 h after the MCAO, one-Way ANOVA, F (2/11) = 4.652, *p = 0.0332 (Bonferroni multiple comparison test) values are min to max, (n for Sham = 4, n for MCAO = 4, n for MCAO + PA = 6), ( C ) PA treatment increased the p38 MAPK at 24 h after the MCAO, One-Way ANOVA, F (2/19) = 9.089, *P = 0.0221 (Bonferroni multiple comparison test), values are min to max, (n for Sham = 4, n for MCAO = 4, n for MCAO + PA = 6). ( D ) PA treatment increased the activity of p38 MAPK 48 h after the MCAO, One-Way ANOVA, F (2/9) = 7.598, *P = 0.0290 (Bonferroni multiple comparison tests), values are min to max, n = 4. ( E,F ) The effect of PA was lost when combining with Triciribine, One-Way ANOVA, F (2/17) = 19.21, **P = 0.0029, ***P = 0.0001 (Bonferroni multiple comparison tests), values are means ± s.e.m, (n for MCAO = 7, n for MCAO + PA = 8, MCAO + PA + Triciribine =5).

Article Snippet: The ELISA of p38 MAPK and GSK 3β was performed using the InstantOne p38 MAPK ELISA kit (Invitrogen, ref. no. 85-86023-11) and InstantOne GSK 3β ELISA kit (Invitrogen, ref. no. 8586173-11).

Techniques: Concentration Assay, Activity Assay

Journal: PLOS ONE

Article Title: Urinary titin is not an early biomarker of skeletal muscle atrophy induced by muscle denervation in mice

doi: 10.1371/journal.pone.0289185

Figure Lengend Snippet: (A) Time course changes in urinary N-titin/Cr levels used #27602 Mouse Titin N-fragment Assay Kit. Open circles: CON group. Closed circles: DEN group. Data are presented as means ± standard error of the mean (SEM). ** p < 0.01 (0 days vs. 1 day after the operation in the CON group) and ### < 0.001 (0 days vs. 1 day after the operation in the DEN group) by one-way repeated measures analysis of variance (ANOVA). (B) Urinary N-titin/Cr values in severe muscular dystrophy models. Values of urinary N-titin/Cr in wild-type and dKO mice. Data are presented as means ± standard error of the mean (SEM). n = 3/group. *** p < 0.001 by an unpaired t -test. (C) Time course changes in urinary N-titin/Cr levels used #SEB667 ELISA Kit. Open circles: CON group. Closed circles: DEN group. Data are presented as means ± standard error of the mean (SEM). (D) Urinary N-titin/Cr values in severe muscular dystrophy models. Values of urinary N-titin/Cr in wild-type and dKO mice. Data are presented as means ± standard error of the mean (SEM). n = 3/group. *** p < 0.001 by an unpaired t -test.

Article Snippet: Next, we used another ELISA Kit (#SEB667 ELISA Kit for Titin, Cloud-Clone Corp.) provided by a different company to test the sensitivity for measuring mouse urinary titin using the same samples.

Techniques: Enzyme-linked Immunosorbent Assay